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rabbit polyclonal anti human grp94  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti human grp94
    Rabbit Polyclonal Anti Human Grp94, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+human+grp94/Grp94+Antibody/pmc09581534-302-62-69
    Average 95 stars, based on 323 article reviews
    rabbit polyclonal anti human grp94 - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: A rapid, automated surface protein profiling of single circulating exosomes in human blood
    Article Snippet: Protein lysates of exosomes (5 μg) were run on 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to PVDF membrane. .. The blots were incubated separately either with mouse monoclonal anti-human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA) at a dilution of 1:10000, mouse monoclonal anti-human CD81 (Cat# NB100–65805, Novus Biologicals, Littleton, CO, USA) at a dilution of 15:10000, rabbit polyclonal anti-human LAMP2B (Cat#ab18529, Abcam, Cambridge, MA, USA) at a dilution of 1:5000, rabbit polyclonal anti-human Grp94 (Cat# 2104P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 5:10000, or with mouse monoclonal anti-human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA) at a dilution of 2.5:10000 (in TBS buffer containing 2% BSA) at room temperature for 1 h followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase, HRP-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison, WI, USA) at a dilution of 1:10000 (2% Milk containing TBS buffer) for 1 h at room temperature.

    Article Title: Extracellular vesicle-based diagnostics and engineered exosomes for targeted therapeutics against cancer
    Article Snippet: Protein lysates of exosomes (5 μg) were run on 4-20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, Calif., USA) and transferred to PVDF membrane. .. The blots were incubated separately either with mouse monoclonal anti-human CD63 antibody (Cat # ab8219, Abcam, Cambridge, Mass., USA) at a dilution of 1:10000, mouse monoclonal anti-human CD81 (Cat # NB100-65805, Novus Biologicals, Littleton, Colo., USA) at a dilution of 15:10000, rabbit polyclonal anti-human LAMP2B (Cat # ab18529, Abcam, Cambridge, Mass., USA) at a dilution of 1:5000, rabbit polyclonal anti-human Grp94 (Cat #2104P, Cell Signaling Technology, Danvers, Mass., USA) at a dilution of 5:10000, or with mouse monoclonal anti-human β-actin (Cat # ab8224, Abcam, Cambridge, Mass., USA) at a dilution of 2.5:10000 (in TBS buffer containing 2% BSA) at room temperature for 1 h followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase, HRP-conjugated goat anti-mouse (Cat # W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison, Wis., USA) at a dilution of 1:10000 (2% Milk containing TBS buffer) for 1 h at room temperature.

    Article Title: Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity
    Article Snippet: Protein- containing lysates of exosomes/EVs (5 μg) were run on a 4–20% Mini- PROTEIN TGX gel (Bio- Rad 4561096) and transferred to a nitrocellulose membrane. .. The blots were incubated separately either with mouse monoclonal anti- human CD44 (156- 3 C11) antibody (Cat# MA513890, Thermo Fisher Scientific, Waltham, MA, USA, RRID:AB_10986810), mouse monoclonal anti- human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA, RRID:AB_306364) at a dilution of 1:1000, Rabbit polyclonal anti- human CD81 (Cat# GTX101766, Genetex, Irvine, CA, USA, RRID:AB_10618892) at a dilution of 1:1000, rabbit polyclonal anti- human Grp94 (Cat# 2104 P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:1000, or mouse monoclonal anti- human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA; RRID: AB_449644) at a dilution of 1:1000 (in Tris- buffered saline [TBS] containing 2% BSA) at room temperature for 1 hr, followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase- conjugated goat anti- mouse [Cat# W402B] or goat anti- rabbit IgG [W401B] from Promega, Madison, WI, USA) at a dilution of 1:10,000 (in 2% BSA containing TBS) for 1 hr at room temperature.

    Article Title: CD81 Facilitates Tumor Cell Clustering and Metastasis in Triple Negative Breast Cancer
    Article Snippet: Tumor-initiating cells with reprogramming plasticity and/or de-differentiation attributes have been thought to initiate primary tumor development as well as to regenerate secondary tumors in metastatic organs; however, the molecular mechanisms are not fully understood.. We previously found that breast tumor-initiating cell marker, CD44, directs multicellular aggregation and cluster formation of circulating tumor cells (CTCs), which further enhance stemness and survival of such cells, enabling metastatic colonization to the lungs.. To further elucidate the molecular network underlying CTC cluster formation, we performed global proteomic profiling and discovered that the tetraspanin protein CD81, which is normally enriched in exosomes (small extracellular vesicles), is a new driver of cancer initiation and metastasis as a facilitator and target of CD44.

    Article Title: CD81 partners with CD44 in promoting exosome biogenesis, tumor cluster formation, and lung metastasis in triple negative breast cancer
    Article Snippet: Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane. .. The blots were incubated separately either with mouse monoclonal anti-human CD44 (156-3C11) antibody (Cat# MA513890, ThermoFisher Scientific, Waltham, MA, USA), mouse monoclonal anti-human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA) at a dilution of 1:1000, Rabbit polyclonal anti-human CD81 (Cat# GTX101766, Genetex, Irvine, CA, USA) at a dilution of 1:1000, rabbit polyclonal anti-human Grp94 (Cat# 2104P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:1000, or mouse monoclonal anti-human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA) at a dilution of 1:1000 (in Tris-buffered saline (TBS) containing 2% BSA) at room temperature for 1 h, followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison, WI, USA) at a dilution of 1:10,000 (in 2% BSA containing TBS) for 1 h at room temperature.

    Article Title: Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity
    Article Snippet: Protein-containing lysates of exosomes/EVs (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad 4561096) and transferred to a nitrocellulose membrane. .. The blots were incubated separately either with mouse monoclonal anti-human CD44 (156-3 C11) antibody (Cat# MA513890, Thermo Fisher Scientific, Waltham, MA, USA, RRID: AB_10986810 ), mouse monoclonal anti-human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA, RRID: AB_306364 ) at a dilution of 1:1000, Rabbit polyclonal anti-human CD81 (Cat# GTX101766, Genetex, Irvine, CA, USA, RRID: AB_10618892 ) at a dilution of 1:1000, rabbit polyclonal anti-human Grp94 (Cat# 2104 P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:1000, or mouse monoclonal anti-human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA; RRID: AB_449644 ) at a dilution of 1:1000 (in Tris-buffered saline [TBS] containing 2% BSA) at room temperature for 1 hr, followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse [Cat# W402B] or goat anti-rabbit IgG [W401B] from Promega, Madison, WI, USA) at a dilution of 1:10,000 (in 2% BSA containing TBS) for 1 hr at room temperature.

    Saline:

    Article Title: Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity
    Article Snippet: Protein- containing lysates of exosomes/EVs (5 μg) were run on a 4–20% Mini- PROTEIN TGX gel (Bio- Rad 4561096) and transferred to a nitrocellulose membrane. .. The blots were incubated separately either with mouse monoclonal anti- human CD44 (156- 3 C11) antibody (Cat# MA513890, Thermo Fisher Scientific, Waltham, MA, USA, RRID:AB_10986810), mouse monoclonal anti- human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA, RRID:AB_306364) at a dilution of 1:1000, Rabbit polyclonal anti- human CD81 (Cat# GTX101766, Genetex, Irvine, CA, USA, RRID:AB_10618892) at a dilution of 1:1000, rabbit polyclonal anti- human Grp94 (Cat# 2104 P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:1000, or mouse monoclonal anti- human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA; RRID: AB_449644) at a dilution of 1:1000 (in Tris- buffered saline [TBS] containing 2% BSA) at room temperature for 1 hr, followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase- conjugated goat anti- mouse [Cat# W402B] or goat anti- rabbit IgG [W401B] from Promega, Madison, WI, USA) at a dilution of 1:10,000 (in 2% BSA containing TBS) for 1 hr at room temperature.

    Article Title: CD81 partners with CD44 in promoting exosome biogenesis, tumor cluster formation, and lung metastasis in triple negative breast cancer
    Article Snippet: Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane. .. The blots were incubated separately either with mouse monoclonal anti-human CD44 (156-3C11) antibody (Cat# MA513890, ThermoFisher Scientific, Waltham, MA, USA), mouse monoclonal anti-human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA) at a dilution of 1:1000, Rabbit polyclonal anti-human CD81 (Cat# GTX101766, Genetex, Irvine, CA, USA) at a dilution of 1:1000, rabbit polyclonal anti-human Grp94 (Cat# 2104P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:1000, or mouse monoclonal anti-human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA) at a dilution of 1:1000 (in Tris-buffered saline (TBS) containing 2% BSA) at room temperature for 1 h, followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse (Cat# W402B) or goat anti-rabbit IgG (W401B) from Promega, Madison, WI, USA) at a dilution of 1:10,000 (in 2% BSA containing TBS) for 1 h at room temperature.

    Article Title: Machine learning-assisted elucidation of CD81–CD44 interactions in promoting cancer stemness and extracellular vesicle integrity
    Article Snippet: Protein-containing lysates of exosomes/EVs (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad 4561096) and transferred to a nitrocellulose membrane. .. The blots were incubated separately either with mouse monoclonal anti-human CD44 (156-3 C11) antibody (Cat# MA513890, Thermo Fisher Scientific, Waltham, MA, USA, RRID: AB_10986810 ), mouse monoclonal anti-human CD63 antibody (Cat# ab8219, Abcam, Cambridge, MA, USA, RRID: AB_306364 ) at a dilution of 1:1000, Rabbit polyclonal anti-human CD81 (Cat# GTX101766, Genetex, Irvine, CA, USA, RRID: AB_10618892 ) at a dilution of 1:1000, rabbit polyclonal anti-human Grp94 (Cat# 2104 P, Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:1000, or mouse monoclonal anti-human β-actin (Cat# ab8224, Abcam, Cambridge, MA, USA; RRID: AB_449644 ) at a dilution of 1:1000 (in Tris-buffered saline [TBS] containing 2% BSA) at room temperature for 1 hr, followed by washing with TBS buffer. .. The blots were incubated with secondary antibody (horseradish peroxidase-conjugated goat anti-mouse [Cat# W402B] or goat anti-rabbit IgG [W401B] from Promega, Madison, WI, USA) at a dilution of 1:10,000 (in 2% BSA containing TBS) for 1 hr at room temperature.



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